Detection of reactive oxygen species (ROS) in mainstream cigarette smoke using fluorescent probe

Non-fluorescence probe dihydrorhodamine 6G (dR6G) can be oxidized to fluorescent rhodamine 6G. In the process, each molecule reacts with two active oxygen molecules. The probe can be used to detect ROS in mainstream cigarette smoke by using derivative fluorescence method. We detected the contents of ROS in mainstream smoke from the 4 types of cigarettes purchased in market. The ROS content is (59.935.32)nmol,(55.986.17)nmol,(54.787.82)nmol, (40.876.43)nmol respectively. The results show that the amount of ROS is dependent on the types of tobacco and cigarette production technology. This realtime ROS detection method in mainstream smoke by fluorescence characteristic is fast and sensitive.