Abstract: In order to achieve real-time monitoring automatically, a controlling system of optical coherence tomography for tissue optical property testing was designed and debugged based on LabVIEW. The system included a programmable driving module for reference arm control and a data acquisition module for data acquisition card operation. The modules were independent and well coordinated. The measurement was executed using different concentrations of Intralipid tissue simulating liquid, and satisfactory results were obtained. The results show that the scattering coefficient of simulating liquid is reliable. The system is cost effective and easy to maintain.