Abstract: The data acquisition in a multi-sensors electro-optical tracker, which includes TV, FLIR, laser rangefinder and fiber gyroscope, was implemented with a digital signal processor and a multi-bus architecture. CPCI bus technique combined with dual port RAM was incorporated to realize high-speed information transmission within the system. It can provide the servo control system with reliable data for fully digitalized control, high-precision sight stabilization, fast and accurate tracking, and improve the performance of the electro-optical tracker effectively. The design scheme and operation principle of the data acquisition system are presented. The control mode and interface design of each sensor in the system are studied. The data transmission between all of the sub-systems is described briefly.