Light-scattering distribution and MCEM model of shape-cells
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Abstract
Based on the application difference in Rayleigh-Debye-Gans theory and CEM model, MCEM (modify cocentral ellipse model) is established to eliminate the influence of the orientation effect of the shape-cell particles on measurement result in light scattering cell particles. Based on the MCEM theory, the variation of the light scattering intensity distribution of cells is discussed systematically at the different incidence angles, the dynamic response relationship between the light-scattering intensity distribution and the relevant physical characteristic of the karyocyte at the different incidence angles was obtained. In addition, the scattering intensity distribution function of the karyocyte is acquired by fitting the scattering intensity distribution. The error analysis shows that the fitting result can be used in the numerical inversion calculation effectively for the light-scattering measurement of the real cell.
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