3D microscopic imaging based on wide-field optical coherence tomography

Wide-field optical coherence tomography（WFOCT） has a promising application for its high scanning rate and resolution. The principle of widefield optical coherence tomography was described, and 2D images of glassware were reconstructed using eight-step phase-shifting method. Besides, the axial resolution and depth detection were studied, and the depth detection could reach 3.3 mm in view of glass material. After obtaining several 2D images, by using VC6.0 and OpenGL programming, the 3D microscopic imaging was realized based on the marching cube(MC) algorithm. The experimental results show that the WFOCT system can be applied in the biological tissues detection as well as the 3D morphology microscopic imaging and depth detection for the objects of high reflectivity.