Abstract: In order to design and debug the stabilizing and tracking control arithmetic of an electro-optical platform system conveniently and efficiently, a hardware-inthe-loop simulation system was designed and implemented based on Matlab xPC target environment. The overall design of the system was presented, and the implementation of software and hardware was described. Through hardware-in-the-loop simulation, the model structure and parameter of the plant were identified, furthermore, proportional integral controller, correction controller, and synthesis controller were designed and tested. Vibration experiments were carried out for every control system designed to evaluate the line-of-sight stabilization accuracy. Experimental results show that the correction controller, which has the maxium gain value in the low frequency band, makes the system achieve the highest stabilization accuracy. According to experimental results, the controller which has optimal disturbance attenuation is selected.