Abstract: The atmospheric wind fields and temperature fields are important atmospherical parameters. The atmospheric wind and temperature measurement technology was already developed in many nations for years, and there is increasingly demanded in China now. Atmospheric wind and temperature are measured by the spectrometers of high spectral resolution, which detects the Doppler line shift and expanding of the special spectrum line in the atmosphere. The in-flight calibration technology is critical for high resolution measurement of the atmospheric wind. Several typical atmospheric wind measurement spectrometers are reviewed, analyzed and summarized. The in-flight calibration technology used in these spectrometers is given.