Abstract: A folding 1∶1 imaging system with switchable waveband and controllable exposure time of 0.1 s～99.9 s was designed. In this system, the front and rear lens groups were connected through a deflection prism, the color filter was changed to achieve dualband imaging, and the high resolution folding lens was coupled with electronic shutter to control the exposure time. The field of view of the optical system was 17 mm, the resolution was enhanced to 8 m，and the working distance reached 55 mm. The results showed that the exposure time and the direction of light could be accurately controlled. The optical system is flexible, costefficient and can be adapted to different lenses according to different requirements in chemiluminescence immunoassay, environmental science and photochemistry.