Abstract: Electro-optical tracking systems are widely used to make the real-time measurement of flying targets in test field due to their capabilities of tracking high speed targets precisely, as well as providing flight path data and flight attitude image in real time. A novel vehicle-borne electro-optical trajectory measurement system is presented. Rigid bearings were used to improve the stability of angular position output. Regenerative feedback technology was applied to suppress lagging error when a high-speed target was tracked. Measurement data with 20 ms update rate were normalized to rectangular coordinates to make data analysis conveniently. Besides, a simulated trajectory detection method to test system in the field is put forward. The system is flexible to be deployed and is able to measure trajectory of flying target promptly and precisely. The steady-state tracking accuracy can reach 0.21 mrad.