Abstract: A microscopic imaging system with a 405 nm blue laser as light source and a 0.65 numerical aperture objective lens was studied. We carried on the microscopic imaging for CD-R and DVD-R discs by using the system, eliminated the laser imaging speckles, observed and stored the microscopic images in real time by utilizing the CCD image sensor technology and the image capturing technology, and compared them with the one using halogen lamp as the light source. The results showed that when this system used the white light of halogen lamp as the light source, the information points on CD-R disc could be clearly resolved, while the image on DVD-R disc couldn-t; however, images on both CD-R and DVD-R discs were clear when the 405 nm laser was used as the light source, and the resolution was better than 400 nm, significantly higher than the ordinary halogen lamp white lighting system.