High-resolution 405 nm laser microscopic imaging
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摘要: 研究了405 nm短波长激光作为照明光源、数值孔径0.65显微物镜组成的显微成像系统。采用该系统对CD-R及DVD-R盘片进行了显微成像，并对激光成像散斑进行了消除，同时利用CCD图像传感技术和图像采集技术对显微图像进行实时观察与存储，与卤素灯白光作为照明光源时的显微图像进行了比较。结果表明：该系统在卤素灯白光作为照明光源时对CD-R盘片信息点可清晰分辨，但对DVD-R盘片信息点的图像不可分辨；而在405 nm激光照明时信息点均可清晰分辨，系统分辨优于400 nm，明显高于普通卤素灯白光照明系统。Abstract: A microscopic imaging system with a 405 nm blue laser as light source and a 0.65 numerical aperture objective lens was studied. We carried on the microscopic imaging for CD-R and DVD-R discs by using the system, eliminated the laser imaging speckles, observed and stored the microscopic images in real time by utilizing the CCD image sensor technology and the image capturing technology, and compared them with the one using halogen lamp as the light source. The results showed that when this system used the white light of halogen lamp as the light source, the information points on CD-R disc could be clearly resolved, while the image on DVD-R disc couldn-t; however, images on both CD-R and DVD-R discs were clear when the 405 nm laser was used as the light source, and the resolution was better than 400 nm, significantly higher than the ordinary halogen lamp white lighting system.
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Keywords:
- high-resolution /
- 405 nm laser /
- CCD image sensor /
- microscope imaging
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