Abstract: Spectral imaging traditional Chinese medicine (TCM) assessing method can be used to evaluate the quality of the medicine. In order to realize the fast, non-destructive and real-time detection and analysis of TCM, software is designed to control the components and take the spectral images. In the system, there are two key components, VariSpec Liquid Crystal Tunable Filter and CCD camera, which can be controlled by the software. The CCD camera obtains a series of spectral images with a specific wavelength interval by controlling the two components simultaneously and automatically. In addition, the software can draw two-dimensional characteristic spectrum curve and spatial distribution curve of three-dimensional of the TCM after processing the original spectral images. The results show that the software is available to control the components to detect the content of the TCM and draw its spatial distribution.