Abstract: High quality reconstruction image of particle fields digital holographic diagnosis can be obtained quickly by high performance reconstruction algorithm. The in-line digital holographic experiment of standard particle plate was designed with a large format CCD, and the large size digital hologram was obtained. The performances of the four reconstruction algorithms, such as the quality of the reconstructed image, the hologram size which can be reconstructed, calculation speed, were studied and compared. The results show that FFT-AS algorithm makes reconstruction image background uniform, without reconstruction image compression, has faster calculation speed and the ability of reconstructing large size holograms, so it is suitable to reconstruct particle fields of in-line digital hologram.