Abstract: The effects of Ag and Fe doping contents on photocatalytic performance of the TiO2 thin film were studied. Ag and Fe doping and co-doping contents on nano-TiO2 photocatalytic bactericidal films were prepared by sol-gel method. The photocatalytic activity of TiO2 films was evaluated by the sterilizing rate of the E.coli. The microstructure and UV absorb ability of films were measured by using XRD and Uv-Vis technology so as to analyze the intrinsic mechanism of Ag and Fe doping the photocatalytic TiO2 thin films. Results show that, for fluorescent light irradiation, optimal doping amounts for Ag/TiO2 and Fe3/TiO2 are 0.05%, 0.1%, respectively. The co-doping of them shows a good photocatalytic effect on the fluorescent light irradiation. And co-doping has a good ability of photocatalytic decomposition. The as-prepared particles had content of anatase phase. By Uv-Vis, we can see co-doping in the visible area has a good absorption property. Fe3+doping expanding the scope of the absorption of TiO2 and Ag+ effecti-cnvely inhibits the photo-generated electron hole recombination. All this makes filmsphotocatalytic ability enhanced. Co-dopeds superiority over the single-doped is to be investigated in the future.