Abstract: Current research status on application of hyper-spectral image technology in the quality of agricultural product and food safety as well as its application in non-destructive detection is reviewed. A method to detect the pesticide residue is provided using hyper-spectral image technology. Its potential application in biomedical information detection and pesticide residue detection is predicted. Hyper-spectral image technology is a new method to biological tissue function information analysis and a potential technology to detect the pesticide residue in agricultural product, which is a serious food safety problem in China.