Abstract: A fluorescence detecting system Based on the opticalelectrical conversion principle of linear array CCD was designed and it was used to measure the fluorescence spectrum of acetamiprid. The CCD detector was used to take place of the photomultiplier which was widely used as the opticalelectrical converter, and the fast analysis of the spectrum was realized by incorporating a grating. Taking the advantage of the complete different features indicated by noise and signal in the case of wavelet transformation, a method for characteristics extraction of pesticide fluorescence signals and noise elimination based on the wavelet transformation theory was proposed. The experimental result shows that the application of the wavelet denoising technology can keep more information at the peak of fluorescence spectrum, and greatly improve the detecting accuracy of the system.