正交偏振光谱微循环成像方法及装置

Method and apparatus of orthogonal polarization spectral microcirculation imaging

  • 摘要: 鉴于微循环图像成像质量的提高是微循环检测仪器实用化的关键,提出一种新的微循环检测方法及装置。利用基于该原理的正交偏振光谱成像仪器进行了实验,得到对比度清晰的甲襞微循环图像。用线性偏振光聚焦照射被测生物组织时,从组织表面反射回来的光保持了其偏振态,被放置在CCD前的偏振方向垂直的检偏器屏蔽掉;从组织内部返回的光发生复散射后退偏,通过检偏器成像。选择光源波长,使其落在红血球的吸收谱范围内,即可得到清晰的生物活体浅表面微循环图像。

     

    Abstract: Since the improvement of microcirculation image quality is the key for the application of such devices, a novel method and apparatus for the microcirculation detection is introduced. The experiments are done with orthogonal polarization spectral imaging apparatus based on this theory and some nail-fold microcirculation images with good contrast are obtained. When linear polarized light is focused on the tissue, the light reflected from the tissue surface remains original polarization state and is blocked by the orthogonal polarization analyzer placed before CCD. The light coming back from the inner of the tissue turns up the multi-scattering and is depolarized. The image is formed after it passes through the analyzer. If the wavelength is selected properly within the absorption spectrum of red cell, clear microcirculation images can be observed.

     

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