Abstract: To detect the wind speed in upper atmosphere at 250km over low and middle latitude area, a scanning FabryPerot interferometer was used to collect the interference images of the nightglow radiation spectral line of OI630 nm at the altitude of 250 km. By analyzing and processing the images, Gaussian function was adopted to match the intensity distribution of interference fringes to get the exact position of intensity center, and to derive Doppler shifts of the spectral line. Then the two vectors of wind in the directions of latitude and longitude were deduced, the effect of the drift vector of the space of FabryPerot etalons on wind velocity was derived by theoretical derivation, and the deduced wind velocity was amended. The result shows that during the measurement period, the longitude wind is towards south, estimated to be 4～67m/s, the latitude wind towards east, estimated to be 20～100m/s, and the error of wind velocity is about 6m/s.