Abstract: Optical coherence tomography(OCT) based on optical reflectance measurement of lowcoherence is a novel imaging technology that performs the noninvasive and highresolution fast imaging of high scattering media such as biological tissue.OCT could result in a significant reduction in the time, complexity and cost for the imaging of developmental morphology as compared with the conventional histology. The noncontact, noninvasion imaging with OCT in vivo can be used to track the development of single speciment.The use of highspeed OCT imaging technology of cardiac structures enables the functional imaging to be performed in vivo of these speciments.OCT imaging is conductive to the developmental research on the variation of function and morphology due to gene mutation. Applications in developmental biology are presented in this paper.