Abstract: By using the virtual instrument technology, non-uniformity correction system of pyroelectric infrared focal plane array (IRFPA) was designed based on LabVIEW software platform. The system can select correction algrithm by using the control panel, and choose three different normal pixels to make different standard correction curves. Moreover,it can be used to acquire the IRFPA pixels- output, test the non-uniformity parameter and correct the non-uniformity. The system can observe the correction contrast with the three-dimensional waveforms and images as well as compute the value of NU (non-uniformity). The non-uniformity correction experiment was done on the 120160 pyroelectric IRFPA video signals. Meaningwhile, the non-uniformity correction algorithms were compared, and the simulation data results were analysed. The feasibility of this system was validated. And it is indicated that the mean algorithm of the twopoint correction is the least for the pyroelectric IRFPA to correct non-uniformity. By the statistical data, it is proved that the value of NU can be averagely decreased by 30%.