Abstract: Under moving condition, the imaging process of optoelectronic system is a dynamic process in a certain period of the integration time. Therefore it needs to invoke the concept of dynamic modulation transfer function (MTF) to evaluate the impact of motion on the imaging quality. By designing a dynamic MTF testing system, the data processing and theoretical analysis for variation of the dynamic MTF were carried out under the moving conditions. Experimental results show that when it is the high frequency vibration, with the increase of the amplitude, the impact of MTF is stronger and the MTF value of the difference is 0.01~0.1 compared with static conditions; when low-frequency vibration, with the reinforcement of vibration frequency as well as the increase of amplitude or integration time, the MTF decreases obviously and the MTF value of the difference is 0.01~0.5 compared with static conditions.