Abstract: To solve the problem of real-time image acquisition and processing in visual measurement, a system, based on NI FlexRIO, was developed with virtual instrument technology by using LabVIEW graphical development platform, in which related techniques, such as ping pong operation, pipelining technique, etc, were applied. The system could implement image capture, storage and processing in parallel and real-time. While the accessed image resolution was 1 2801 024 and the sampling rate was 500 f/s, the system worked stably. In the process, the time of image processing was less than 2 ms, and the precision could reach the level of sub-pixel. The system is significant for visual measurement to be implemented in real-time, and it is also highly valuable in other relative fields for its good performance.