Abstract: Based on the background of distributed optoelectronic system, the problems and deficiencies in the use of equipment were analyzed, and a dynamic target calibration scheme for installing inertial navigation system was proposed in distributed optoelectronic system. By adopting the method of transfer alignment, the distributed optoelectronic system and aircraft alignment calibration were completed. Firstly, the operational background of distributed optoelectronic system and the significance of transfer alignment were analyzed, the working principle of transfer alignment and dynamic target calibration were briefly expounded, the algorithm design of transfer alignment of distributed optoelectronic system was analyzed, and the sports car test of transfer alignment algorithm was completed. The test results demonstrate that the convergence time is better than 3 min, and the alignment accuracy is better than 0.1°, which meets the requirements of dynamic target calibration of the airborne distributed optoelectronic system.