Abstract: To meet the technical requirements of high sensitivity, high resolution and low noise of high-resolution spectrometer, the back-illuminated CCD driving circuit and main control circuit for low-level-light imaging system were designed. The MAX X series FPGA of Altera was adopted by linear CCD acquisition system as the core control device to provide multiple driving signals for the linear CCD. The output analog signal of linear CCD was transformed into digital signal after signal preprocessing and AD sampling, and then sent to the spectrometer through USB interface module. By installing the linear CCD acquisition system into the high-resolution spectrometer to test the characteristic peaks of the mercury lamp spectrum, the spectral resolution can reach 0.062 nm, which meets the detection requirements of high-resolution spectrometer.