Abstract: In the optical imaging system, the dynamic modulation transfer function (DMTF) is affected by the wave aberration of optical system, the signal resolution and transfer characteristics of detector modules, and the image blur caused by the carrier motion, which has become one of the important parameters of the photoelectric imaging system. Based on the testing principle of dynamic and static modulation transfer function of photoelectric imaging system, a DMTF measuring device was developed, in which the focal length of optical collimation system was 10 000 mm, and the speed control range of moving target was 30 mm/s~5 000 mm/s, which met the DMTF test of photoelectric imaging system with long focal length. A series of experiments were carried out, and the results show that the repeatability of DMTF measurement is better than 0.01, and the uncertainty of measurement can reach to U= 0.05 (k=2).