Abstract: In order to measure the cell traction more quickly and intuitively, a measurement method based on Fourier transform was proposed. Firstly, the two-dimensional fast Fourier transform was applied to the image of the micro-column array to obtain its spatial spectrum. Then, one spot in the first-order diffraction spot was selected for the fast Fourier transform to obtain the amplitude distribution of the micro-column array. Finally, the distribution of the offset of the micro-column array could be obtained from the distribution of amplitude mutation and the severity of mutation, and then the distribution of cell force could be obtained by multiplying the stiffness of the micro-column. The influence of frequency point selection, filter window size and offset point size on the experimental results was investigated through simulation experiments. On this basis, the high-power micrograph of cell micro-column was measured. The experimental results show that this method is comparable to the centroid method in terms of the result presentation. The relative error of the maximum cell force measured in each region is within 17.37%, the relative error of the average cell force measured in each region is within 7.93%, and the operation speed is nearly 10 times faster than that of the centroid method.