Abstract: UV-VUV imaging spectrometer has been widely used in space exploration in China. It needs to be calibrated in its development process. However, there is no corresponding measurement standard in China, which can not guarantee the accuracy and reliability of the measurement results. Therefore, a calibration device and method of UV-VUV imaging spectrometer was designed to calibrate the parameters of UV-VUV imaging spectrometer, such as spectral range, wavelength, spectral responsivity, spatial angular resolution, uniformity, etc. The results show that the uncertainty of wavelength measurement is 0.08 nm ( k=2), the uncertainty of spectral responsivity measurement is 6.8% ( k=2), the uncertainty of spatial angular resolution measurement is 0.022 mrad ( k=2), and the uncertainty of uniformity measurement is 4.2% ( k=2).