Abstract: The UV (ultraviolet) image intensifier is the core part of the missile UV warning system, UV early warning system and the various UV radiation monitoring systems, and the accuracy of its parameters directly affects the image quality of the system. In order to ensure the accuracy of the test data, an resolution calibration device of the UV image intensifier was developed. The UV light source used in the resolution calibration device of the UV image intensifier was the UV light with a wavelength range of 200 nm to 400 nm. The corresponding resolution targets, filters, and the optical imaging systems were all required the ability to transmit the UV light. Due to the short wavelength of the UV light, it was easy to generate a great deal of stray light caused by the scattering effect. The UV quartz was adopted by the designed resolution target, the transmission structure was adopted by the UV optical imaging system, and the coaxial conjugate lens was selected as the UV optical imaging system. The experiment and the measurement uncertainty analysis verify that the measurement uncertainty of the calibration device is 5%.