Abstract: Modulation transfer function (MTF) is an important parameter to evaluate the imaging performance of TV cameras. A measuring device and method for MTF of visible TV camera were introduced. A three-integral spherical target generator system was designed, the target and background brightness can be independently controlled by electric attenuators. The outlet non-uniformity is 4.1%. An automatic sub-pixel interpolation algorithm based on sliting slit method and a least square method of line spread functions were wtilized to realize the angle calculation automaticly and MTF measurement accurately. The repeatability of the measuring device is 0.007, and the measurement uncertainty is U=0.05 (k=2). Finally, the comparison experiment with foreign advanced instruments shows that, the measurement device established can effectively solve the problem of evaluating the image quality of TV cameras.