Abstract: The dedicated bus testing system is based on single-item, which leads to poor versatility and compatibility, has difficulties of function expansion and technical upgrade. A design scheme of measurement system which can support 5 kinds of avionics standards like 1553B, ARINC429, CAN, RS232 and RS422 is introduced, as well the technology implementation. The hardware framework of the data bus test system is introduced emphatically. The system software realization method and the bus logic control design are also analyzed. The test system is used for on-line detection of electro-optical(EO) equipments, and experiments verifies that the system is stable and reliable.