Abstract: The optical axis parallelism of the spatial rotating multi-optical axis system affects the pointing accuracy of the system and is difficult and time consuming to calibrate.Based on the optical axis calibration principle of the spatial rotating multi-optical axis system, the calibration theoretical model was obtained. Combined with the experimental research, a high-precision optical axis calibration scheme was established. Taken the mechanical rotation axis as the reference target, the coarse and fine adjustments were combined, the coarse adjustment was performed for the sensor mounting surface and the fine alignment of optical magnitude was implemented by means of double wedges. Firstly, the parallelism between the visible light axis and the mechanical rotary axis was calibrated, then the parallelism between the laser axis and the mechanical rotary axis was guaranteed, and finally the parallelism between the visible light axis and the laser axis was guaranteed. The experimental results show that the calibration scheme is accurate and efficient, the index is better than 0.1 mrad, which can be used for practical engineering.