Research on extended field depth of wavefront coding microscope objective
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摘要: 为了解决传统显微物镜景深与分辨率的矛盾，采用波前编码的方法，设计研究了波前编码的10倍显微物镜，结合传统光学设计软件，采用基于MTF一致性的相位板参数的优化方法，实现了在像面附近一定范围内系统的点扩散函数一致性；此外，还设计了扩展三次相位编码板的10倍显微物镜，比较了采用两种不同相位板系统的焦深扩展的效果，结果显示三次相位板的焦深扩展效果较好，可以将传统10倍显微物镜的焦深扩展15倍。成像模拟仿真结果显示滤波后的编码像在±15倍焦深范围内成像清晰，从而扩大了系统的景深。Abstract: In order to solve the contradiction between the depth of field and the resolution of traditional microscope objective, the wavefront coding(WFC) 10× microscope objective was designed and researched based on WFC technique. Combining with traditional optical design software, an optimization method for cubic phase mask parameters based on modulation transfer function (MTF) consistency was used to achieve the point spread function(PSF) consistency of the system within a certain range near the image plane. In addition, a 10× microscope objective lens with an extended cubic phase mask was also designed to compare the effect of the depth-of-focus expansion using two different phase mask systems.Design results show that the cubic phase mask has a good focal depth expansion effect, which can extend the focal depth of the traditional 10× microscope objective by 15 times. Simulation and experimental results indicate that the encoded images of system are sharp within ±15 times depth of focus distance after filtering so as to extend the field depth of system.
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Keywords:
- microscope objective /
- depth-of-focus expansion /
- imaging system /
- wavefront coding
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图 8 扩展三次相位板系统离焦MTF(彩图在网上)
Figure 8. Defocus MTFs of extended cubic phase mask (color online)
表 1 显微物镜的结构参数
Table 1 Parameters of 10× microscope objective
照明光源 f′/mm NA 物高/mm 理论焦深/μm 可见光 12 0.18 ±2.8 ±4.696 
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