Abstract: At present, with the structural complexity and performance requirements of military optical lenses increasing, the defects of great errors and inconsistency in the conventional adjustment methods become more and more obvious. Therefore, a precise axis centering calibration technology for optical lens was proposed, in order to providing support for advanced optical systems. First of all, the error sources affecting the quality of optical lens were analyzed. Second, the manufacturing chain principle with data feedback closed-loop was proposed, and the entire optical system development links were combined together into a closed loop. Then, aiming at the shortcomings of the existing adjustment methods, a precise calibration method of lens group axis taken centering process as the core was put forward through ensuring both the center deviation and the optical spacing. Its optical-mechanical axis consistency reaches the second level and the dimensional accuracy reaches the micron level. Finally, a set of optical lens was designed and developed using this technique. Compared with the traditional lens, the focal length error increases from 1% to 0.1% and the resolution from 2.3" to 2.04".