Abstract: In order to develop the laser remote sensing ways for the acquisition of ocean optical parameters, a new method was presented to measure the ocean internal optical parameters using subsurface backscattering signal of lidar, based on the application of high spectral resolution lidar (HSRL) in the measurement of atmosphere backscattering. In theory,the system building and the method of separation measurement of the backscattering signal from water molecules (Rayleigh scattering) and suspended particles (Mie scattering) using iodine molecules absorption filter were introduced. In result, the specific inversion algorithm for calculating the backscattering coefficient and the extinction coefficient of the sea water was presented.