Abstract: In order to validate the feasibility that the intensified charge-coupled device (ICCD) detect target by polarization laser assistant illumination, a polarization laser assistant illumination ICCD detection system was manufactured by polarization imaging detection technology. The system adopted an 808 nm semiconductor laser as illumination source to illuminate target, which wasn-t detected by human-eye, and the ICCD polarization detection system was used as electric-optic conversion module to detect target, and finally achieved the target detection on background. An experiment was designed to validate the result of the polarization imaging detection in the field environment. The result shows that the man-made-metal-target depolarization is almost 10%, and the green-background-target depolarization is 50%, and the sand-target depolarization is almost 30% 0 at 45, and 90 polarization angle. The imaging contrast for the man-made target and the natural background is improved as the polarization angle enlarging.