Optical design of near infrared fluorescence confocal laser scanning system
-
摘要: 为了实现对近红外荧光的高分辨率扫描，设计了工作在近红外光谱区的激光共聚焦光学系统。采用结构简单的凹凸双透镜物镜实现了照明光路和发射光路的设计，并采用Zemax软件进行了光学设计和仿真。实验表明：照明光路的聚焦弥散斑小于1 m，照明针孔处的聚焦光斑小于40 m，满足照明针孔的尺寸要求；发射针孔处的聚焦光斑小于10 m，满足探测针孔尺寸要求；同时照明光路和发射光路的MTF曲线的截止频率都分别满足其衍射极限分辨率的要求，照明光路在全视场空间分辨率420 lp/mm处MTF0.08，发射光路在全视场空间频率400 lp/mm处MTF0.07。Abstract: In order to realize the high resolution scanning for near infrared fluorescence，a laser scanning confocal optical system was designed which worked in the near infrared region.The lighting lens,and emission lens were realized based on simple concave lens and convex lens.The software Zemax was used for the optical design and simulation. Simulation results shows that the focal spot-s size of lighting lens is less than 1 m, and the focal spot-s size is less than 40 m at the lighting pinhole position, which meets the lighting pinhole-s requirement.The focal spot-s size is less than 10 m at the emission pinhole position, which meets the emission pinhole-s requirement.In addition,the cutoff frequency of the lighting lens and emission lens meets the diffraction limit resolution requirement respectively. Therefore, it has high optical transmission efficiency.
-
-
[1]Duan Xia.Synthesis and application of NIR fluorescence dye for biological analysis[D].Jinan:Shandong Normal University,2009.
段霞.用于生物分析的近红外荧光染料的合成与应用[D].济南：山东师范大学，2009.
[2]Huo Xia,Lyu Jianxun,Yang Rendong,et al.Comparison of laser xcanning confocal microscope with light microscope[J].Acta Laser Blology Sinica,2001,10(2):76-79.
霍霞，吕建勋，杨仁东，等.激光共聚焦显微镜与光学显微镜之比较[J]. 激光生物学报，2001，10（1）：76-79.
[3]Hu Maohai,Yang Xiao-chun.High-speed laser scanning control system for confocal microscopy[J].Journal of Applied Optics,2011,32(4):797-800.
胡茂海，杨晓春.共聚焦显微镜激光高速扫描控制系统设计及实现[J].应用光学,2011,32(4):797-800.
[4]Fan Yingjuan，Zhang Yanjun..Design of He-Ne laser beam focusing lens based on Zemax[J]. Journal of Applied Optics,2010,31（6）：1032-1035.
范应娟，张艳军.基于Zemax的He-Ne激光光束聚焦物镜的设计[D]. 应用光学，2010,31（6）：1032-1035.
[5]Lin Xiaoyang.Super manual of ZEMAX optical design[M].Beijing:Posts & Telecom Press,2014.
林晓阳.ZEMAX光学设计超级学习手册[M].北京:人民邮电出版社,2014.
[6]Liu Jun，Gao Ming.Optic design[M].Xi-an:Xi-an University of Electronic Technology Press,2006.
刘钧，高明.光学设计[M].西安：西安电子科技大学出版社，2006.
[7]Xiao Zexin.System and application of optical mechanical and electrical integration[M].Guangzhou:South China University of Technology Press,2011.
萧泽新.光机电一体化系统及应用[M].广州：华南理工大学出版社， 2011.
-
期刊类型引用(4)
1. 孙景旭，费强，谢虹波，王硕，谢新旺. 真空低温高灵敏度中红外辐射计. 红外与激光工程. 2023(11): 127-136 . 
百度学术
2. 杨科，程刚，郭羽，周根东，卢飞，李辉，刘建平，孙宇楠，宫经珠，张旭，吴李鹏，张清，孙帅，郭建. 精密红外辐射计测量模块研究. 应用光学. 2022(04): 738-743 . 本站查看
3. 艾葳. 宽动态红外辐射测量精度的二次修正方法研究. 信息技术与信息化. 2021(11): 123-125 . 百度学术
4. 边悦，邱添，蕫银杏. CT球管阳极靶盘循环加热除气装置研制. 真空科学与技术学报. 2020(11): 1085-1089 . 百度学术
其他类型引用(1)
计量
- 文章访问数: 1831
- HTML全文浏览量: 119
- PDF下载量: 128
- 被引次数: 5
下载:
陕公网安备 61011302001501号 